2DYT

The crystal structure of Saccharomyces cerevisiae Atg3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 41
Unit cell lengths	59.116, 59.116, 115.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.840 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.24400
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.271
Number of reflections	83016
<I/σ(I)>	22.3
Completeness [%]	98.8	99.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.3M ammonium sulfate, 1.0M lithium sulfate, 0.1M citrate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K