2DYO
The crystal structure of Saccharomyces cerevisiae Atg5- Atg16(1-57) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 73.253, 73.253, 148.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.730 - 1.970 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.24500 |
Structure solution method | MIR, MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.055 | 0.304 |
Number of reflections | 29390 | |
<I/σ(I)> | 28.9 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 15% PEG 3350, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |