2DYN

DYNAMIN (PLECKSTRIN HOMOLOGY DOMAIN) (DYNPH)

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	86.020, 86.020, 137.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.310 - 2.300
R-factor	0.2139
Rwork	0.214
R-free	0.29660 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT WITH ANOMALOUS SCATTERING
RMSD bond length	0.006
RMSD bond angle	23.215 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.310	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.144
Number of reflections	11759
<I/σ(I)>	6.5	5.1
Completeness [%]	99.5	99.3
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1		PROTEIN (10-20 MG/ML) WAS CRYSTALLIZED FROM 10-30% PEG 8000, 0.2 M AMMONIUM SULFATE, PH 6.1 BY THE HANGING DROP VAPOR DIFFUSION METHOD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20-30 (%)
2	1	reservoir	ammonium sulfate	0.2 (M)