2DYK
Crystal structure of N-terminal GTP-binding domain of EngA from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.421, 50.539, 50.453 |
| Unit cell angles | 119.88, 90.02, 88.91 |
Refinement procedure
| Resolution | 43.810 - 1.960 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mky |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.020 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 20112 | |
| <I/σ(I)> | 14.9 | 2.56 |
| Completeness [%] | 92.0 | 78.6 |
| Redundancy | 1.91 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH 6.0, 1.0M Pottasium sodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
