2DYC

Crystal structure of the N-terminal domain of mouse galectin-4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-07
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9790, 0.9794, 0.970
Spacegroup name	I 4 2 2
Unit cell lengths	92.053, 92.053, 132.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 2.400
R-factor	0.22
Rwork	0.220
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	11598
<I/σ(I)>	17.2
Completeness [%]	99.8	100
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.4M Ammonium Sulfate, 12% Glycerol, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K