2DYB

The crystal structure of human p40(phox)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	146.273, 189.813, 79.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 3.150
R-factor	0.263
Rwork	0.263
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6h 1w6x and 1OEY
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.071
Number of reflections	22612
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	5% PEG 20000, 200mM Bis-tris-HCl, 10mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K