2DY0
Crystal structure of project JW0458 from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-06 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 40.106, 48.192, 54.795 |
Unit cell angles | 109.40, 101.84, 110.56 |
Refinement procedure
Resolution | 35.000 - 1.250 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1ZN9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.057 | 0.244 |
Number of reflections | 88230 | |
<I/σ(I)> | 9.9 | 3.2 |
Completeness [%] | 94.6 | 91.9 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbach | 8.5 | 291 | 30 w/v(%) PEG 4000, 0.1M Tris, 0.2M Mg Chlor , pH 8.5, Microbach, temperature 291K |