2DXM
Neutron Structure Analysis of Deoxy Human Hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | NUCLEAR REACTOR |
| Source details | JRR-3M BEAMLINE 1G-B |
| Synchrotron site | JRR-3M |
| Beamline | 1G-B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-20 |
| Detector | MACSCIENCE |
| Wavelength(s) | 2.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.790, 84.450, 54.350 |
| Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| Rwork | 0.197 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Deoxy Human HB X-ray model(unpublished) |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.500 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.099 |
| Number of reflections | 21841 |
| Completeness [%] | 70.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | D2O, MICRODIALYSIS |
