2DXA

Crystal structure of trans editing enzyme ProX from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-04-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.686, 53.812, 65.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.970 - 1.580
R-factor	0.18
Rwork	0.180
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dbx
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.970	1.640
High resolution limit [Å]	1.580	1.580
Number of reflections	20627
<I/σ(I)>	50.2
Completeness [%]	98.1	91.2
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG3350, Tris, 10mM Cys-SA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K