2DX8
Crystal Structure Analysis of the PHD domain of the Transcription Coactivator Pygophus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.286, 1.2826 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.079, 60.079, 95.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.19924 |
| Rwork | 0.197 |
| R-free | 0.25495 |
| Structure solution method | MAD |
| RMSD bond length | 0.032 |
| RMSD bond angle | 1.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.093 | 0.270 |
| Number of reflections | 5038 | |
| Completeness [%] | 96.5 | 81.9 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 1.2M Na Citrate, 0.2M LiSO4, 0.1mM ZnCl2, 50mM Tris, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
