2DX6

Crystal structure of conserved hypothetical protein, TTHA0132 from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	93
Detector technology	CCD
Collection date	2005-06-23
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9791, 0.9798, 0.970
Spacegroup name	C 1 2 1
Unit cell lengths	149.967, 30.522, 62.263
Unit cell angles	90.00, 110.46, 90.00

Refinement procedure

Resolution	31.060 - 1.780
R-factor	0.195
Rwork	0.195
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.870
High resolution limit [Å]	1.780	1.780
Number of reflections	25601
<I/σ(I)>	27	10.57
Completeness [%]	98.9	98.9
Redundancy	3.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	26% PEG3350, 0.1M Na Acetate, 6% iso-propanol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K