2DX0
Crystal structure of the N-terminal SH2 domain of mouse phospholipase C-gamma 2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9794, 0.9700 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 80.905, 80.905, 133.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15954 | |
| <I/σ(I)> | 22.8 | 7.8 |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | ammonium sulfate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
