2DWU
Crystal Structure of Glutamate Racemase Isoform RacE1 from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.783, 49.659, 198.252 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.162 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gzm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.506 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.620 | |
| High resolution limit [Å] | 1.530 | 3.220 | 1.530 |
| Rmerge | 0.053 | 0.044 | 0.214 |
| Number of reflections | 105445 | ||
| <I/σ(I)> | 22.74 | 38.7 | 5.6 |
| Completeness [%] | 83.7 | 99.8 | 42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 298 | 0.2M potassium fluoride, 20% PEG 3350, 10mM yttrium chloride, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
