2DWP

A pseudo substrate complex of 6-phosphofructo-2-kinase of PFKFB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-12
Detector	MAR CCD 165 mm
Wavelength(s)	1.3808
Spacegroup name	P 65 2 2
Unit cell lengths	101.990, 101.990, 258.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.251
Rwork	0.224
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2axn
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.790
High resolution limit [Å]	2.700	2.700
Number of reflections	21941
Completeness [%]	96.4	64.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 4000, ETHYLENE GLYCOL, TRIS-PHOSHATE, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K