Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-27 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9792, 0.9795, 0.9640 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 86.523, 86.523, 106.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.590 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16483 | |
| <I/σ(I)> | 14.5 | 5.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 0.05M SODIUM CHLORIDE, 1.4M AMMONIUM SULFATE, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
