2DWA
Structure of the complex of lactoferrin C-terminal half with fucose at 2.07 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2006-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5414 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.365, 50.383, 65.814 |
Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.070 |
R-factor | 0.17549 |
Rwork | 0.174 |
R-free | 0.20931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nkx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.412 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.140 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 23077 | |
<I/σ(I)> | 11.8 | 2 |
Completeness [%] | 94.8 | 93.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, temperature 298K |