2DVY
Crystal structure of restriction endonucleases PabI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.570, 113.956, 89.173 |
| Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.31800 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.400 | 3.000 |
| Rmerge | 0.067 | 0.035 | 0.283 |
| Number of reflections | 30368 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.3 | 99.8 | 96.7 |
| Redundancy | 3.6 | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 5% PEG 6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
