2DVP
Structure of NTPase from Pyroccous horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-02-28 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 71.516, 71.516, 78.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.27623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v7r |
RMSD bond length | 0.013 |
RMSD bond angle | 1.325 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.046 | |
Number of reflections | 16658 | |
<I/σ(I)> | 20.8 | |
Completeness [%] | 96.2 | 99.3 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 295 | NaCl, acetate, pH 4.6, microbatch, temperature 295K |