2DUQ
Crystal structure of VIP36 exoplasmic/lumenal domain, Ca2+/Man-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2005-11-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.200, 45.000, 117.000 |
Unit cell angles | 90.00, 131.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2duo |
RMSD bond length | 0.011 |
RMSD bond angle | 1.334 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.099 | 0.282 |
Number of reflections | 59750 | |
<I/σ(I)> | 9.1 | 2.9 |
Completeness [%] | 96.1 | 84.5 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP & SOAKING | 6.5 | 277 | 15% PEG4000, 1.5M NaCl, 0.1M MES (pH6.5), 50mM mannose, VAPOR DIFFUSION, HANGING DROP & SOAKING, temperature 277K |