2DUM
Crystal structure of hypothetical protein, PH0823
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-17 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97921, 0.9795, 0.964 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 120.786, 120.786, 127.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.750 |
| R-factor | 0.21867 |
| Rwork | 0.216 |
| R-free | 0.27918 |
| Structure solution method | MAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 20726 | |
| <I/σ(I)> | 14.5675 | 1.86869 |
| Completeness [%] | 87.2 | 30.88 |
| Redundancy | 5.83957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 28% Pentaerythritol Ethoxylate (5/4 PO/OH), pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
