2DUE
crystal structure of a green fluorescent protein variant S65T/H148D at pH 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.887, 62.590, 69.911 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.240 |
R-factor | 0.1547 |
Rwork | 0.148 |
R-free | 0.20400 |
Structure solution method | AB INITIO |
RMSD bond length | 0.013 |
RMSD bond angle | 0.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Number of reflections | 59945 | |
Completeness [%] | 93.7 | 61.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 298 | 100mM MgCl2, 100mM CHES pH 10, 26% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |