2DUD
Crystal structure of human mitochondrial single-stranded DNA-binding protein(hmtSSB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 106.900, 106.900, 90.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.990 - 2.700 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3o |
| RMSD bond length | 0.067 |
| RMSD bond angle | 4.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 8832 | |
| <I/σ(I)> | 68.4828 | 11.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.5 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | CHES, 0.2M NaCl, 10% PEG 8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
