2DTS
Crystal Structure of the Defucosylated Fc Fragment from Human Immunoglobulin G1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.472, 77.690, 143.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.19483 |
| Rwork | 0.192 |
| R-free | 0.25064 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3x |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.593 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.669 |
| Number of reflections | 28426 | |
| <I/σ(I)> | 10.2 | 2.6 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 277 | Deionized water, pH 7.0, MICRODIALYSIS, temperature 277K |
