2DT9

Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2005-11-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 43 3 2
Unit cell lengths	141.773, 141.773, 141.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 2.150
R-factor	0.197
Rwork	0.197
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.067
Number of reflections	27145
<I/σ(I)>	59.9	11.1
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M sodium acetate trihydrate, 1.4M sodium chloride, 5mM threonine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M sodium acetate trihydrate, 1.4M sodium chloride, 5mM threonine, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K