2DSW

Binding of chitin-like polysaccharides to protective signalling factor: crystal structure of the complex of signalling protein from sheep (SPS-40) with a pentasaccharide at 2.8 A resolution

Replaces:  2B2PReplaces:  2AOK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-07-25
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.660, 66.480, 107.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.800
R-factor	0.199
Rwork	0.199
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dpe
RMSD bond length	0.009
RMSD bond angle	1.700
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	11764
<I/σ(I)>	8.5	2
Completeness [%]	99.3	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	25MM TRIS-HCL, 50MM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K