2DSV

Interactions of protective signalling factor with chitin-like polysaccharide: Crystal structure of the complex between signalling protein from sheep (SPS-40) and a hexasaccharide at 2.5A resolution

Replaces:  2B2LReplaces:  2ANF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-07-05
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.770, 66.310, 107.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.540
R-factor	0.187
Rwork	0.187
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dpe
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.580
High resolution limit [Å]	2.540	2.540
Number of reflections	14762
<I/σ(I)>	7.6	2.3
Completeness [%]	97.2	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	25MM TRIS-HCL, 50MM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K