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2DSV

Interactions of protective signalling factor with chitin-like polysaccharide: Crystal structure of the complex between signalling protein from sheep (SPS-40) and a hexasaccharide at 2.5A resolution

Replaces:  2B2LReplaces:  2ANF
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU300
Temperature [K]298
Detector technologyIMAGE PLATE
Collection date2005-07-05
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameP 21 21 21
Unit cell lengths62.770, 66.310, 107.122
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution56.000 - 2.540
R-factor0.187
Rwork0.187
R-free0.22700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2dpe
RMSD bond length0.008
RMSD bond angle1.700
Data reduction softwareAUTOMAR
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]56.0002.580
High resolution limit [Å]2.5402.540
Number of reflections14762
<I/σ(I)>7.62.3
Completeness [%]97.294.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.829825MM TRIS-HCL, 50MM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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