2DS0
Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-27 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.435, 87.684, 41.384 |
| Unit cell angles | 90.00, 117.36, 90.00 |
Refinement procedure
| Resolution | 22.880 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2D12 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 22875 | |
| Completeness [%] | 96.2 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 20% PEG8000, 0.2M sodium chloride, 0.1M phosphate-citrate, 15mg/ml 6'-sialyllactose, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
