2DRU
Crystal structure and binding properties of the CD2 and CD244 (2B4) binding protein, CD48
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 100 |
Collection date | 1999-02-16 |
Wavelength(s) | 0.977 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 96.780, 96.780, 126.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.200 - 2.600 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ccz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 9426 |
Completeness [%] | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M sodium HEPES, 1.5M lithium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |