2DRT
Structure Analysis of (POG)4-LOG-(POG)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 14.092, 24.748, 84.449 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| Rwork | 0.159 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.021 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.085 | 0.316 |
| Number of reflections | 7797 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 98.6 | 99.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 12.5% PEG 400, 0.05M acetate buffer, 5.0% Ethanol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
