2DRR
Crystal structure of reducing-end-xylose releasing exo-oligoxylanase D263N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.182, 86.235, 87.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.230 - 1.600 |
R-factor | 0.1627 |
Rwork | 0.162 |
R-free | 0.18164 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1wu4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.157 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.430 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 53617 | |
<I/σ(I)> | 27.9 | 3.1 |
Completeness [%] | 99.4 | 98.8 |
Redundancy | 5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG4000, SODIUM ACETATE, GLYCEROL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |