2DRM
Acanthamoeba myosin I SH3 domain bound to Acan125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Wavelength(s) | 0.86 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.969, 37.861, 44.506 |
| Unit cell angles | 90.28, 90.11, 90.64 |
Refinement procedure
| Resolution | 23.640 - 1.350 |
| R-factor | 0.18678 |
| Rwork | 0.186 |
| R-free | 0.20626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2drk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.204 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.300 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.020 | |
| Number of reflections | 44074 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 89.4 | 53.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 280 | pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
