2DR9
Complex structure of CCA-adding enzyme with tRNAminiDCC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.858, 57.858, 429.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.990 - 2.800 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 17014 | |
Completeness [%] | 100.0 | 60.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 297 | 50mM HEPES, 20% PEG 3550, 0.2M Tri-lithium citrate, 80mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, temperature 297K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | HEPES | ||
10 | 1 | 2 | water | ||
2 | 1 | 1 | PEG 3550 | ||
3 | 1 | 1 | Tri-lithium citrate | ||
4 | 1 | 1 | ammonium sulphate | ||
5 | 1 | 1 | water | ||
6 | 1 | 2 | HEPES | ||
7 | 1 | 2 | PEG 3550 | ||
8 | 1 | 2 | Tri-lithium citrate | ||
9 | 1 | 2 | ammonium sulphate |