2DQX
mutant beta-amylase (W55R) from soy bean
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.835 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.042, 85.042, 145.083 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 2.200 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wdp |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.074 | |
Number of reflections | 31087 | |
Completeness [%] | 98.9 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 0.1M sodium acetate (pH 5.4), 1mM EDTA, 50% ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |