2DQV
Structure of the C-terminal lobe of bovine lactoferrin in complex with galactose at 2.7 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-24 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.149, 50.463, 65.966 |
| Unit cell angles | 90.00, 107.64, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g93 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 10794 | |
| Completeness [%] | 97.4 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE, GLYCOLMONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
