2DQR
Crystal structure of the replication terminator protein mutant RTP.E39K.R42Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.922, 72.805, 195.008 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.450 - 3.010 |
| R-factor | 0.20532 |
| Rwork | 0.202 |
| R-free | 0.27626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bm9_A (residues 72 to 88 omitted) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.570 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.142 | 0.690 |
| Number of reflections | 12062 | |
| Completeness [%] | 98.8 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 22% PEG 4000, 0.2M sodium acetate, 0.225M Tris HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
