2DQM
Crystal Structure of Aminopeptidase N complexed with bestatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.417, 120.417, 170.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.182 |
| R-free | 0.19400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2dq6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 187171 | |
| <I/σ(I)> | 18.8 | 3.9 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 6.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | the crystal is soaked in 2.2M ammonium sulfate, 0.1M MES pH 6.4, 1mM bestatin. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
