2DQL

Crystal structure of the circadian clock associated protein Pex from anabaena

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	50.126, 75.040, 103.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.234
Rwork	0.234
R-free	0.25500
Structure solution method	MIR
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.660	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.065	0.484
Number of reflections	41503
<I/σ(I)>	7.5	1.7
Completeness [%]		94.7
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	282	1M Sodium Phosphate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 282K
1	VAPOR DIFFUSION, HANGING DROP	4.8	282	1M Sodium Phosphate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 282K