2DQ6

Crystal Structure of Aminopeptidase N from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	120.480, 120.480, 170.791
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.181
R-free	0.19100
Structure solution method	MIR
RMSD bond length	0.004
RMSD bond angle	1.290
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.046	0.299
Number of reflections	228266
<I/σ(I)>	55.2	4.5
Completeness [%]	100.0	100
Redundancy	9.5	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	297	1.75M ammonium sulfate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	6.4	297	1.75M ammonium sulfate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K
1	VAPOR DIFFUSION, HANGING DROP	6.4	297	1.75M ammonium sulfate, 0.1M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K