2DPZ
Structure of the complex of phospholipase A2 with N-(4-hydroxyphenyl)- acetamide at 2.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.063, 53.063, 48.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.20531 |
| Rwork | 0.205 |
| R-free | 0.21701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fb2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.696 |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 7815 | |
| Completeness [%] | 98.5 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.2M ammonium sulphate, 30% PEG 4000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
