2DPY
Crystal structure of the flagellar type III ATPase FliI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.160, 72.750, 125.740 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 45.980 - 2.400 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.29500 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.238 |
| Number of reflections | 33960 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 8% PEG 8000, 0.1M calcium acetate, 3% MPD, 0.1M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
