2DPW
Hpothetical Transferase Structure from Thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97894, 0.97933, 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.190, 76.190, 196.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.090 - 2.900 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.581 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | SOLVE (2.10) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.090 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.084 | 0.331 |
| Total number of observations | 16205 | |
| Number of reflections | 8150 | |
| <I/σ(I)> | 25.8 | 10.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.05 | 20.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.2 | 293 | Lithium sulfate 1.2M, Sodium Acetate 0.1M, pH 5.2, VAPOR DIFFUSION, temperature 293K |
