2DPQ
The crystal structures of the calcium-bound con-G and con-T(K7gamma) dimeric peptides demonstrate a novel metal-dependent helix-forming motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2003-05-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 29.334, 29.334, 46.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.250 |
R-factor | 0.119 |
Rwork | 0.119 |
R-free | 0.16100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 14-mer polyalanine helix |
RMSD bond length | 0.010 |
RMSD bond angle | 0.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.081 | 0.923 |
Number of reflections | 12427 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 98.0 | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 35% dioxane, pH 8, VAPOR DIFFUSION, HANGING DROP |