2DPD
Crystal structure of the Replication Termination Protein in complex with a pseudosymmetric B-site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 117.006, 117.006, 165.553 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 3.170 |
| R-factor | 0.23835 |
| Rwork | 0.236 |
| R-free | 0.27781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F4K with residues 71 to 89 of each monomer omitted |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.718 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 11638 | |
| <I/σ(I)> | 23.2 | 3.3 |
| Completeness [%] | 97.5 | 91.6 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% MPD, 75mM sodium acetate pH 4.6, 20mM calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | calcium chloride | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | sodium acetate | ||
| 7 | 1 | 2 | calcium chloride | ||
| 8 | 1 | 2 | HOH |
