2DPD
Crystal structure of the Replication Termination Protein in complex with a pseudosymmetric B-site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 117.006, 117.006, 165.553 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.000 - 3.170 |
R-factor | 0.23835 |
Rwork | 0.236 |
R-free | 0.27781 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1F4K with residues 71 to 89 of each monomer omitted |
RMSD bond length | 0.013 |
RMSD bond angle | 1.718 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 3.250 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 11638 | |
<I/σ(I)> | 23.2 | 3.3 |
Completeness [%] | 97.5 | 91.6 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% MPD, 75mM sodium acetate pH 4.6, 20mM calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | calcium chloride | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | sodium acetate | ||
7 | 1 | 2 | calcium chloride | ||
8 | 1 | 2 | HOH |