2DP8

Carbohydrate recognition by lactoferrin: Crystal structure of the complex of C-terminal lobe of bovine lactoferrin with trisaccharide at 2.5 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2006-04-29
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	63.082, 50.437, 65.790
Unit cell angles	90.00, 107.53, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.18471
Rwork	0.184
R-free	0.20034
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2B6D
RMSD bond length	0.010
RMSD bond angle	1.646
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	12749
Completeness [%]	91.6	83.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M MES, 25% POLYETHYLENE, GLYCOLMONOMETHYL ETHER 550, 0.01M ZINC SULPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K