2DP5
Structure of streptococcus pyogenes bacteriophage-associated hyaluronate lyase Hylp2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5414 |
Spacegroup name | H 3 2 |
Unit cell lengths | 59.587, 59.587, 588.546 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.550 |
R-factor | 0.22273 |
Rwork | 0.221 |
R-free | 0.23625 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c3f |
RMSD bond length | 0.012 |
RMSD bond angle | 1.773 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.650 |
High resolution limit [Å] | 3.550 | 3.550 |
Number of reflections | 5360 | |
<I/σ(I)> | 4.7 | 1.9 |
Completeness [%] | 98.1 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Tris HCl, Sodium formate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |