2DOU

probable N-succinyldiaminopimelate aminotransferase (TTHA0342) from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-06
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	121.490, 121.490, 172.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.300
R-factor	0.222
Rwork	0.222
R-free	0.25400
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	1109831
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Ammonium sulfate, PEG 8000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K