2DOR

DIHYDROOROTATE DEHYDROGENASE A FROM LACTOCOCCUS LACTIS COMPLEXED WITH OROTATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1995-11
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	53.910, 109.030, 67.070
Unit cell angles	90.00, 104.43, 90.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.161
Rwork	0.161
R-free	0.18700
Structure solution method	DIFFERENCE MAP
RMSD bond length	0.011
RMSD bond angle	23.813 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.061 *	0.250 *
Total number of observations	179226 *
Number of reflections	50309
<I/σ(I)>	18.7	3.6
Completeness [%]	99.3	90.5
Redundancy	3.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		PROTEIN WAS CRYSTALLISED FROM A SOLUTION CONTAINING 30% PEG 6000, 0.2M SODIUM ACETATE AND 0.1M TRIS-HCL, PH 8.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	30 (%)
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
4	1	drop	protein	18 (mg/ml)