2DOO
The structure of IMP-1 complexed with the detecting reagent (DansylC4SH) by a fluorescent probe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.517, 88.416, 115.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.430 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.880 | 2.510 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 19418 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | HEPES, SODIUM ACETATE, SODIUM-CITRATE, PEG 4000, PH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |