2DN3
1.25A resolution crystal structure of human hemoglobin in the carbonmonoxy form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.7000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 53.270, 53.270, 189.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.250 |
R-factor | 0.183 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 0.028 |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.320 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.061 | 0.533 |
Number of reflections | 76777 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 6.7 | 293 | sodium/potassium phosphate, glycerol, pH 6.7, BATCH, temperature 293K |