Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.7000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.105, 82.914, 53.650 |
| Unit cell angles | 90.00, 99.29, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.250 |
| R-factor | 0.179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a3n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.067 | 0.465 |
| Number of reflections | 145478 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 97.0 | 93.8 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 6.5 | 293 | Ammonium sulfate, Ammonium phosphate, pH 6.5, Batch, temperature 293K |
